Journal: Cell metabolism

Article Title: Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-α-Dependent Intensification of Oxidative Stress and Tumor Cell Death

doi: 10.1016/j.cmet.2018.05.012

Figure Lengend Snippet: (A) Detection of ROS in cultured CT26 cells. CT26 cells were subjected to the indicated culturing conditions for 18 hr. Mafosfamide (maf) or melphalan (mel) was used as chemotherapy. Cells were stained with CM-H2DCFDA and DAPI to evaluate ROS levels and cell viability, respectively. Gating on live cells (DAPI−), histograms show overlay of CM-H2DCFDA curves under different culturing conditions. The MFIs of CM-H2DCFDA are summarized in bar graphs and shown as mean ± SEM.

Article Snippet: Murine colorectal cancer cell line CT26 (ATCC), mammary carcinoma 4T1 (ATCC) and B-cell lymphoma cell line A20 (ATCC) were cultured in RPMI 1640 (HyClone Laboratories) supplemented with 10% fetal bovine serum albumin (FBS), 1% penicillin/streptomycin (HyClone Laboratories), 1% non-essential amino acids and 1% glutamine (Corning) at 37°C in a 5% CO2 incubator.

Techniques: Cell Culture, Staining

Journal: Cell metabolism

Article Title: Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-α-Dependent Intensification of Oxidative Stress and Tumor Cell Death

doi: 10.1016/j.cmet.2018.05.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Murine colorectal cancer cell line CT26 (ATCC), mammary carcinoma 4T1 (ATCC) and B-cell lymphoma cell line A20 (ATCC) were cultured in RPMI 1640 (HyClone Laboratories) supplemented with 10% fetal bovine serum albumin (FBS), 1% penicillin/streptomycin (HyClone Laboratories), 1% non-essential amino acids and 1% glutamine (Corning) at 37°C in a 5% CO2 incubator.

Techniques: Recombinant, Activation Assay, Lysis, Binding Assay, Transfection, Luciferase, GSH Assay, SYBR Green Assay, Gel Extraction, Plasmid Preparation, Cell Isolation, Mouse Assay, CRISPR, Software

Journal: Scientific Reports

Article Title: Bioactivity of crude fucoidan extracted from Sargassum ilicifolium (Turner) C. Agardh

doi: 10.1038/s41598-022-19370-7

Figure Lengend Snippet: Fucoidan of S. ilicifolium from different locations that was extracted at ( a ) 65 °C and ( b ) 80 °C on the cell viability of the L929 ( a ) ( b ), HCT116 ( c )( d ) and A549 ( e )( f ) cell lines. Bars indicate SD, n = 3. (** P < 0.01 and * P < 0.05).

Article Snippet: HCT116 (human colorectal carcinoma) cells were purchased from ATCC, and cultured in McCoy's 5A medium that was supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% antibiotics (AA) in an incubator with 5% CO 2 at 37 °C.

Techniques:

Journal: Scientific Reports

Article Title: Three-dimensional culture models mimic colon cancer heterogeneity induced by different microenvironments

doi: 10.1038/s41598-020-60145-9

Figure Lengend Snippet: 3D cultures of colorectal cancer cell lines LoVo and LS174T. LoVo cells ( a–c ) or LS174T cells ( d–f ) were adapted to SCM and used for 3D cultures. ( a,d ) Growth of the 3D cultures was analysed with CellTiter-Glo. Data represent mean + s.d. (n = 6). ( b,e ) Representative whole mount immunostaining images of phalloidin (white) and DAPI (blue) in cultures on Day 13. Scale bars = 100 μm. ( c,f ) Expression of CSC ( LGR5 , CD44 , PROM1 ) and differentiation ( CDX1 , CDX2 , KRT20 ) markers by quantitative RT-PCR analysis. With LoVo cells, cultures on Day 10 and 13 were examined for stemCO and diffCO culture conditions, respectively. With LS174T cells, cultures on Day 13 were examined for both culture conditions. Data represent mean + s.d. (n = 3). *p < 0.05, Student t test.

Article Snippet: The colorectal cancer cell lines LoVo and LS174T (both from ATCC) were grown in F-12K (Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS) or Eagle’s MEM supplemented with 1% Non-Essential Amino Acids Solution, 1 mM Sodium Pyruvate (all from Thermo Fisher Scientific), and 10% FBS, respectively, at 37 °C under 5% CO 2 .

Techniques: Immunostaining, Expressing, Quantitative RT-PCR